I want to work on honeybee hemocyte.How can I prevent honeybee haemolymph from coagulation for long time?(60-120 min)
did you used any coagulant?. try it
Hi Ali, there was a thread on RG regarding insect hemolymph coagulation, I hope it helps.
https://www.researchgate.net/post/How_can_i_prevent_insect_hemolymph_from_coagulation
Usually we use heparin. It works with the hemolymph in cockroaches and hemipterans
Try the addition of 200 mM (final concentraiton) arginine, pH 8.
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