Seed surface sterilization with 20% NaOCl, washing with sterile distilled water few times and then experimentation.

Thanks too much

rightly said Branislav.

Sometimes, contamination comes from microorganisms present in your extracts, so, in addition to sterilize the seeds, you can try to filter the extracts.

Thanks for all

You must sterilize the seedss using sodium hypochlorite and keep all equipments sterile

Use distilled water and filter extract.

1- Seed surface sterilization with 20% NaOCl

2- Use distilled water and filter extract

You can use the chloroform vapor to sterilize each of the seeds and plant extracts