I am going to scan seeds by NIR spectroscopy. However, since there is some resin in berries, I would like to know how to clean off the resin from the seeds and which solvent I should use so that it doesn't influence the spectra?
I am using n-hexane for oil removing from oil rich seeds. You should try several solvents or solvents systems in order to achieve best results.
Hi,
The residues of solvent could affect the spectra. But why do you want to remove the resin? Most of the seeds can be scanned as such by NIRS even without grinding. Which seeds are involved?
Pierre
Thanks for your comments. I am scanning Juniper seeds but I am afraid that the seeds covered by resin have different spectra. I mean I would like to clean the surface of the seeds off the resin.
If you need only to know how the spectrum from seeds looks like, you can mechanical collect some resin , take a resin spectrum and subtract it from the spectrum of "impurity" seeds.
Alternativ, you can use the same treatment protocol for all your measurements. Although the solvents affect the resulting spectra it will be done on the same way in all experiments.
Great Answer, Gennadi. Thank you. As you recommended I will firstly scan the resin as a reference , and then start scanning the seeds.Excellent!
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