I also need these informations. I am searching some papers. If i find anything about this subject, I will share here.

Aspergillus grows well in media containing glucose, yeast extract and some minerals and vitamins. Cellulase are extracellular, so you can centrifuge broth to collect supernatant containing desired enzyme and discard the cells.

Refer to publications e.g.  New Biotechnology. 2009 Sep;25(6):437-41. doi: 10.1016/j.nbt.2009.02.002. Cellulase production from Aspergillus niger MS82: effect of temperature and pH.

Try to optimise the media composition, time for maximum enzyme activity.

You will need shaker, flask, laminar hood, autoclave, enzyme analysis method with equipments like spectrophotometer, GC or HPLC to analyse ethanol and sugars respectively.

Dear Rezania,

              Preparing cellulase from Aspergillus niger is quite interesting. There are so many literatures available in sciencedirect and microsoft academic search. Once if you gothrough you will get the complete protocol of the production and purification of cellulase enzyme. Once the enzyme purified from ammonium sulphate precipitation or through ion exchange chromatography, you can use the crude enzyme and check the activity before and after purification process.

You are using dried biomass of Water Hyacinth. It should be completely dried, sieved and make it as a powder. Follow similar procedure as mentioned in the paper which I have attached.

you are using simultaneous saccharification and fermentation, better check the saccharification efficiency of separate hydrolysis and fermentation rather than simultaneous saccharification and fermentation.

Instruments as our friend Shrikant Dhoot already told, apart from that, confirmation of enzyme activity, better do cellulase assay and DNS method for determining the reducing sugar efficiency. Then go for HPLC or GC for ethanol determination.

Check the attachment for details and further information.

With regards,

Ramadoss

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