Hello, good day! I am a student and I am currently working on a project that requires the preparation of a 0.5 wt% chitin suspension (200 mL) with the pH adjusted to 3, preferably using glacial acetic acid. I would greatly appreciate guidance on the recommended preparation protocol, including dispersion techniques, order of addition, and any tips to improve suspension stability. References to recent studies or standard methods would also be helpful.
To prepare a 0.5 wt% chitin suspension (200 mL) at pH 3, disperse 1 g chitin slowly into dilute acetic acid solution adjusted to pH 3 with magnetic stirring, followed by optional mild sonication for better stability. Store at 4°C and gently re-disperse before use to maintain homogeneity.
Hello, thank you Abdelhak Maghchiche. Would you happen to have any recommendations for recent research papers, review articles, or standard protocols that describe similar preparation methods? Literature references would be greatly appreciated so I can study the methodology in more detail and ensure my procedure aligns with established practices.
Angel Ramonica Mingote Jimanga I am not sure you can do that. Chitin is not like chitosan. It doesn't dissolve in weak acid. Check this reference: Chapter Solubility of Chitin: Solvents, Solution Behaviors and Their...
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