I want to use this to run RAPD in the lab
Hello
Try this calculator online
http://www.biomol.net/en/tools/buffercalculator.htm
Hi,
You can use this sheet for preparation of 10 mM Tris-HCl
https://dharmacon.horizondiscovery.com/uploadedFiles/Resources/crrna-tracrrna-resuspension-buffer-protocol.pdf
Moles of Tris =(desired morality M or mol/L of your final sol ) * [ L (final volume of your solution) ]
= ** mole
g of Tris = Moles of Tris * (121.14 g/mol)
= ** g
Add the calculated grams of TRIS in DI water (take 80% volume of water e.g for 100 ml solution use 80 ml water )
Add HCL or NACL to achieve desired PH level
& then add the DI water to get to its final volume
I am performing NDVI anomaly analysis in ArcMap using MOD13Q1. After flagging out the contaminated pixels some data sets have NDVI values ranging from 0 to 0. How do I correct this?
27 August 2020 1,387 1 View
As part of my research study, I have been trying to make my result representation for measured and predicted values for Cesium-137 depth distribution pattern. However, i find it difficult to plot...
11 December 2019 4,216 2 View
In scaling-up laboratory technologies, what are some of the areas to consider throughout the demonstration stages in order to get the technology to the commercial application?
08 September 2019 9,818 1 View
how to measure government official, NGOs tourist.
11 December 2018 4,333 0 View
Dear Researchers, Please share the details you look out for when analyzing data/ or raw results from experiments (both qualitative and quantitative). How do you make your discussion sections look...
10 November 2018 816 6 View
Want to run a cross tab for 2 categories of multiple responses. First category is Times of the day i.e Dawn to Midnight and the second category is Destinations (School, Work, Home, etc). But I...
07 June 2018 6,887 2 View
please how best can DNA from excised gel be stored for 2 weeks? thank you
18 July 2017 2,580 3 View
10 November 2016 1,343 0 View
Hi everyone. I want to know the current research issues (areas) in Security in WSNs. I am specifically looking at DOS attack at the various layers of WSNs or Intrution Detection in WSNs. All...
20 October 2016 9,305 3 View
09 October 2016 2,302 4 View
I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.
03 March 2021 5,997 2 View
When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...
03 March 2021 4,892 5 View
Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...
03 March 2021 2,350 2 View
I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.
02 March 2021 4,359 3 View
Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !
02 March 2021 5,785 3 View
Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...
02 March 2021 7,829 3 View
So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...
02 March 2021 1,146 2 View
Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...
01 March 2021 8,235 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...
01 March 2021 4,128 3 View