NaOH +CO2 give rises to  NA2CO3+ H2O

Na2CO3+ Hcl give rises to NaCl + H2CO3

H2CO3 give rises to by decomposition H2 + CO2

The color of phenophthalene depends on its ph value, Up to 8.3, it is color less;

In the range of 8.3-14,  it in pink in color.  

Thanks a lot Dr. Maddli ..But could you please sent me the procedure of CO2 and Ch4 emissions determination from Tritraion method ....on my mail [email protected]

Regards

amit 

CO2 of soil is measured by alkali absorption technique.

After trapping CO2 in NaOH you should add BaCl2 to get a stable precipitate before titration with HCl. Usually, 20 mL 0.25 M BaCl2 is added to 10 mL aliquots of trapping solution.

CH4 must be converted to CO2 by combustion. However, for this you will need a specific oven (oven with quarz glass tube (catalysator) at 850°C). After the addition of oxygen it is catalytically (= ZnO) oxidized in an oven at 600°C - 900°C to form CO2. The formed CO2 is trapped in the CO2-trap situated at the outlet of the oven.

Thiele-Bruhn...sir could you please tell me the final colour after titration with HCL as well as Determination of CH4 from titration method..............

The color changes from purple/pink to colorless.

pH 0–8.2 colorless; pH 8.2–12.0 pink to purple.

You stop titration at the point where the pink color has disappeared.

Again, the determination of CH4 works by combustion to CO2.

Then you can identify it using the same alkali absorption technique.

Thiele-Bruhn Soren....Myriad of thank for your positive reply...Could you please send me the procedure to determine CH4 in soil...at minimum facility level ..Yet gain, i am waiting for your positive reply in expected lines...

Regard 

amit 

Dear Amit, 

If you get the protocol for determination of CH4 by titration method. Kindly forward the detail procedure of it on my mail-id

[email protected]

Thank a lot.. 

Sorry, we don't have such an oven either.

You will have to check the literature.

Regards STB

Dear Amit,

  I dont know if this is going to help you but the methane cannot be obtained using titration. First you need to trap methane and then you need to have an analysis which i have not seen so far. People around the world use few sensors that are designed specially for the GHG measurement from soil. You may find these equipments at IARI, Delhi. As far as CO2 is concerned i will mail you the calculation and the procedure using titration.

no sir, you have to create anaerobic condition by purging with N2 gas or remove air from bottle...see the above discussion with Thiela Bruhn regarding CH4....he has given good reply ..but i want more..thanks for your concern too..

Hi sir,

The issue with titration method for CO2 determination is that NaOH solution (freshly prepared 0.01N) itself when directly titrated wih dil.HCl (0.05N) seems to bring a colour change. How is this possible? What could be the reason for this observation?

if you look at my papers under the title of Response of microbial community in the arid land to wheat residues

and soil compaction

Salwan AL-Maliki*

or Effect of tillage and no-tillage on aggregate stability, organic matter content and microbial activity

in the Babylon province, Iraq

Salwan M. AL-Maliki*,

you will find a decent information regarding to soil respiration as I am spent a fun time with it.

Basal Respiration by static alkali method

20 g of soil was placed inside a flask corked with a stopper to which a 10 ml glass bottle was

attached. The bottle was filled with 5 ml solution of 0.62M NaOH. The NaOH solution captured

any CO2 that was respired from the soil. 1

4

0.5 M HCl was used to neutralize 5 ml of NaOH containing a phenolphthalein indicator, the

addition of three drops of the indicator into the NaOH solution caused a colour change to pink. The

endpoint of the titration was when the pink colour was changed to pale. The amount of carbon

dioxide evolved in soil respiration was estimated based on( 36).




− (B-V)

N22



where B = standard HCl used to titrate NaOH in the blank (mL), V =standard HCl used to

titrate NaOH in the treatment (mL), N = normality of HCl (1.00 N), 22 = equivalent weight

of CO2, W = dry weight of soil in the chamber (g).

Dear Colleague,

I trust this message finds you well. Your interest in performing soil CO2 and CH4 analysis through the titration method is both relevant and challenging, given the complexities associated with accurately quantifying gaseous components in soil samples. Here, I present a structured approach to address this analytical task, focusing on the indirect measurement of CO2 as a more feasible target for titration methods. Please note, directly quantifying CH4 by titration is significantly more complex and generally not practical due to its non-reactive nature in standard titration scenarios. For CH4, gas chromatography remains the gold standard. However, I will focus on CO2 for the purpose of this guidance.

Background:

Quantifying soil CO2 through titration involves capturing CO2 in a liquid absorbent and subsequently determining the absorbed CO2 amount via titration. This method is based on the principle that CO2 can react with water to form carbonic acid, which then can be titrated with a base.

Materials Required:

• Soil sample
• Sodium hydroxide (NaOH) solution, typically 0.1 M, as the CO2 absorbent
• Hydrochloric acid (HCl) solution, typically 0.1 M, for titration
• Barium chloride (BaCl2) solution, to precipitate carbonate ions
• Phenolphthalein indicator
• Airtight container for soil incubation
• Gas collection system (e.g., a gas-tight syringe or a balloon attached to the incubation container)
• Erlenmeyer flasks
• Burette

Protocol Overview:

Considerations:

• The accuracy of this method depends on the efficiency of CO2 capture and the precision of the titration process. Calibration with known standards is recommended to validate the method.
• Environmental factors, such as temperature and soil moisture, significantly influence microbial respiration rates. Consistent conditions during incubation are crucial for reproducible results.
• This method primarily quantifies CO2 production rates, offering insights into soil microbial activity rather than the total soil carbon content.

Conclusion:

While direct titration methods for soil CH4 analysis pose significant challenges, quantifying soil CO2 through the described indirect titration approach can provide valuable information on soil microbial respiration and carbon cycling dynamics. For methane, exploring alternative methods such as gas chromatography will be necessary.

Should you require further guidance or clarification on this procedure, please feel free to reach out.

Best regards,

Reviewing the protocols listed here may offer further guidance in addressing this issue