Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

How should I compute a new variable with z-scores?

I would like to create a new variable using 4 different measures: hIndex (range: 1-47), # of publications (range: 0-111), and 2 other measures (range: 1-7). I changed all of these variables into...

09 February 2021 4,866 3 View

Can cell lines have different DNA content?

I am doing calibration curves using CyQUANT Direct Assay to 10 different pancreatic cancer cell lines. I've noticed that the fluorescence of some cell lines double at the same cell number. Is this...

03 February 2021 3,587 2 View

Have you cloned a PCR product containing 2X2A sequences into a vector that contains another 2A sequence?

Hi! I am having problems cloning a 1.6 kb fragment containing 2 different genes and 2X2A peptides into a vector that I already used to clone other fragments (digested with PmeI/BamHI). The vector...

17 December 2020 5,218 3 View

How to migrate a graph database to SQL?

In the research that I am working on, I need to migrate a graph database to sql to carry out tests, I am using neo4j as a tool, the information I have found is about migrating from SQL to neo4j...

09 November 2020 1,869 3 View

Can I incubate two secondary antibodies at the same time for western blot?

I want to know if I can use a goat-anti mouse IgM-HRP and a goat-anti mouse IgG-HRP to treat a western blot membrane at the same time. Thank you

26 September 2020 1,332 6 View

How to obtain Uranium metal from Uranyl Nitrate?

I got some Uranyl Nitrate crystals and want to get Uranium metal, to start, think I can use heating to decompose the nitrate salt in order to get the oxide, and then I should reduce the oxide to...

22 August 2020 525 3 View

Is it dangerous for me to handle Ethyl methanesulfonate (EMS) in an open air space?

18 June 2020 3,918 3 View

What are the most common data file types used to create Virtual Reality environments?

I know most of the 3D file types used in VR but I could be missing out on some that are more commonly used and would like to see what others are using. Also any ideas on what file types relating...

19 February 2020 3,207 3 View

Is there any free software similar to WinNonlin?

I want to predict a toxicocinetic model of some molecules. I have windows and linux.

17 September 2018 7,412 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

No title

01 March 2021 3,905 5 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

How to highlight novelty in your research paper?

I have come up with a method and had written a paper on it. Paper got rejected by a journal stating that there is no theoretical novelty. I am kind of confused on why the novelty that I am able to...

28 February 2021 2,393 8 View

Can I follow Researchers from Teaching and testing areas?

I want Researchers from Educational Measurement and Evaluation, relating to teaching, learning , academic performance and test validation

18 February 2021 4,757 13 View

Do you know of an informal caregiver of a person who has recently received a bone marrow transplant?

We are conducting a pilot study of an intervention for informal caregivers of persons who have recently received a bone marrow transplant in the US. If you know of anyone who might be interested,...

13 February 2021 9,174 1 View

Which and How to use teaching strategies and procedures to effectively integrate the four skills while teaching English Language Online?

As an inexperienced teacher in the field of online teaching, I have the fear of unconsciously ignoring one of the skills while focusing on not ignoring the other! Therefore, I wish to make use of...

09 February 2021 5,101 4 View

Best analysis method to use for my variables?

I need to conduct an analysis and wanted to check if my idea for analysis sounds feasible. I have 3 DVs which are; fear of terrorism, confidence in the police and punitive attitudes towards...

07 February 2021 5,746 10 View

Is receiving Invitation to submit a article from the journal is big deal ???

I have recently received a invitation from the journal "Current Pharmaceutical Bio-technology" to submit a research article. As i didn't received any invitation before. so, is it normal to to...

04 February 2021 4,596 23 View

Which is the appropriate statistic test to examine the relation between students' affective state and academic performance?

Which test should I use to compare students' mood effects on academic performance? I want to compare mood measured on a likert scale (from point 1= very sad to point= 5 very happy) to three...

04 February 2021 1,907 5 View