We are now evaluating camel serum level of vitamins A, D and E. We are in need to know how to measure and any available kits.
Dear DR.Mohamed
The same procedure for human analysis is good way to do the job, but the problem is that hospitals or labs will not accept doing such analysis.
α-Tocopherol and Retinol assay
For the measurements of α-Tocopherol and Retinol, You have to deproteinized plasma with ethanol that contains the internal standards (α-tocopheryl acetate and retinyl acetate), and the lipid is extracted with hexane. After an aliquot of the solvent phase is evaporated, the residue is dissolved in diethyl ether and diluted with methanol. A portion of this solution is injected onto a C18 reversed-phase chromatographic column, and absorbance of the vitamins and internal standards are measured at 280 nm. Peak-height ratios are used to quantify each vitamin (Catignani and Bieri, 1983).
I hope this article helping you
http://www.jofamericanscience.org/journals/am-sci/am0801s/017_16106am0801s_106_111.pdf
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