You can use Atomic Absorbsion instrument or other insturementel analaysis.You can find them in a food control lab easily I think.

Have nice day,

Erdem

you could use ICP - MS

For extermely accurate results and high sensitivity your best bet would be to find a facility that has Atomic Absorption or ICP-MS. There are several colormetric assays on the market that should work but they detect from the low micromolar to low millimolar range.

Atomic absorption and ICP gave best results, there is also chemical methods after Trichloroacetic acid filtration. The method is explained in details in the following link.

http://www.jbc.org/content/90/3/747.full.pdf

Mahmoud El tawila

www.cord.edu/dept/chemistry/CCJAC/2010_Vol_1/2010_1_1.pdf

go through dis site..there is test to check the mg levels

you can find all methods in AOAC

I agree with upper post. Atomic absorption instrument is the best choice for measuring but it's a lot cost.

I am afraid but I don t work on this area. Sorry

You can find the details in AOAC official methods. Earlier worked on sodium, potassium and Iron using atomic absorption spectrometer.

I don't work in this area though i know, that Atomic Absorption spectometer would be most accurate and appropriate.

I agree with Lawrance Gray, and Mahmoud El Tawila. I attach a file containing the method for Ca, Mg and P estimation in Milk. Hope it can be modified for using in urine also.

Atomic absorption and ICP WILL give best results

in this link its clear

http://www.jbc.org/content/90/3/747.full.pdf

Atomic absorption could be an interesting method, but there are also the posibility of Mg electrode, or a automatic analyzer (in a clinical biochemistry lab).

Josephine Kasolo

It is Atomic absorption spectroscopy which is used on biological materials.

magnesium level can be measured by flame photometric method using magnesium nitrate as a reference standard. A flame photometer instrument is extremely simple where the sample in solution is aspirated through an aspirator or nebulizer into the flame which is usually a propane / air fuel or, even, a purified natural gas/air mixture. The sample matrix evaporates followed by atomization of the sample. Atoms present in the high temperature zone of the flame are excited to higher energy levels by absorbing energy from the flame. As excited atoms return to the ground state they emit radiation in definite wavelength depending on the energy level from which each atom drop. This gives rise to a line spectrum. However, in flame photometry a pre-selected filter (depending on the atom in question) is used and it is the intensity of the emission line that is practically measured and is related to the original concentration of the sample in solution. The detector is usually a phototube or a photomultiphier tube depending on the quality of the instrument.

Atomic emission spectrometry (AES) or inductively coupled plasma atomic emission spectrometry (ICP-AES) would be suitable to analyse milk or urine. For solutions and materials with low concentratrions of the analyte better results can be obtained by ICP-AES. You might be able to get your material analysed at your local university geology, earth science or environmental science laboritory .

You can find your answer in this sites

http://paras.tbzmed.ac.ir/common/201083113759.pdf

http://www.sarmadteb.com/content.asp?id=6&t=tbl_articles

I agree with the all colleagues Atomic emission spectroscopy (AES) or mass spectroscopy ICP-MS could be the best sutable method to mesurement Mg and I guess that a previous digestion by microowave oven will be need necessary.

Trichloroacetic acid method can be adopted to find out the magnesium levels

40 cc of milk pippeted out into a 200 cc volumetric flask. Fill it till 200 cc mark with 10% trichloroacetic acid solution slowly by rotating flask constantly and mixing thoroughly. Allow to stand for 30 minutes. Filter using 15 cm acid washed filter paper with funnel covered to avoid evaporation. 150 cc of aliquorts portion corresponding to 30 cc are used. 30 cc samples are used for ashing. Follow the same procedure that as for ashing

You can follow the method describes by AOAC. However, Atomic absrption spectrophotometry, or ICP-MS is the best method to be used.

You can determine it using AAS after digesting the organic matrix with hot acid. If you want detail protocol I may prepare one for you

Thanks all, I really wanted a simple easy to do method I can use in the field, literally.

I am working with a herd of mares and foals and needed something easy to do.

All these other methods need a lab.

Thank you though.

MW

I guess you can do biochemical analysis of milk and urine to find out the Magnesium level.

As you know metel determination suffer a lot interference, you most to eliminate interfering agent(s) for accurate estimation of metal contents. using simple field method, without sample preparation may not echo well with your aim.

For milk I agree with Catherene Isaac, while for urine you must work with

HCl treated 24-hour urine to prevent precipitation. In my past job I made magnesium measure with these below reagents:

Buffer reagent: dissolve in approximately 800 ml dH2O, 93.6 g 2-amino-2-methyl-1-propanol, 4.0 g caffeine, 0.5 g thiourea, 50.7 g KCl, adjust pH at 11.6 using 1 M HCl, and then bring volume to 1.0 liter using dH2O.

Dye reagent: dissolve in abaut 800 ml dH2O, 0.072 g calmagite, 0.083 g EGTA, add 100 ml of dimethylsulfoxide, adjust pH to 6.0 using 1 M HCl, and then bring volume to 1.0 liter using dH2O.

Working reagent: mix equal quantities of the buffer reagent and the dye reagent. The reagent thus prepared has a pH of about 11.3 and will retain its usefulness for several months or longer.

Manual procedure

Dispense 1.0 ml of the working reagent and 10 µl of the sample mix, and after one minute, the Abs of the reaction mixture is measured between 540 and 550 nm. For calibration, a sample or calibrator or standard, containing a known amount of magnesium is similarly treated. To calculate the amount of magnesium in the sample, divide the Abs of the unknown by the Abs of the calibrator, then multiply the result thus obtained by the known amount of magnesium in the calibrator