You have drug X and dissolve it in DMSO at a concentration of 100 mg / mL. Your goal is to create an aliquot of this drug with a final concentration of 200ug/mL. The solvent you will use to dilute it is cell culture media. Is it possible to create this aliquot while keeping DMSO below 0.1% v/v? I have tried this and could not get it below 0.2%. If anyone can please help and show how they arrived at the answer I would really appreciate it!
Yes Mike we cannot reach the concentration of DMSO below 0.1% with a Stock solution of 100 mg / ml.
According to my calculation, if you increase the concentration of the drug to 200 mg/ml , you can achieve the DMSO concentration of 0.1%
The only way to achieve the
Yes Mike we cannot reach the concentration of DMSO below 0.1% with a Stock solution of 100 mg / ml.
According to my calculation, if you increase the concentration of the drug to 200 mg/ml , you can achieve the DMSO concentration of 0.1%
The only way to achieve the
1. Dissolve drug at >200mg/ml stock.
2. Otherwise, dissolve drug directly into media containing
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