Hello everyone, I’m a master’s student working on an experiment to transfect the pGL4 NF-kB luciferase plasmid into the Jurkat cell line.

Initially, I used Lipofectamine and Fugene 4k following both literature and product protocols, but the transfection was unsuccessful.

I then achieved successful transfection with the Neon Transfection System (Life Technologies). However, luciferase expression is only detectable for a short period of about 24 hours, even when applying a selection marker.

Has anyone encountered a similar issue or found a solution?

Any insights would be greatly appreciated!

Thank you!