I have to make lacZ- revertants of E. coli MG1655. I first need a strain which is lacZ-. How can I find a strain with this genotype on the Coli Genetic Stock Center (CGSC)?
Are there any suggestions or insights you can provide to help address this problem
06 August 2024 1,443 2 View
Why knockouts for same genes from two different gRNA are showing different result? I followed CRISPR-CAS9 gene editing technology.
25 July 2024 8,313 1 View
While doing AST for Pseudomonas aeruginosa, after incubation, no zone of inhibition observed in the plate near the well. wells surrounded by bacterial growth, when the same plate observed under UV...
25 July 2024 9,229 1 View
I want to write article in a topic of positive role of politics in nursing, and I'm confused how can we add add questionnaire and what will be the questionnaires.
18 July 2024 10,021 3 View
We are trying to coat some cathode materials on Al-foil ( ex - LiFePO4 , S@CNT, etc). When we coated the Al foil with the cathode (ex - S@CNT) material , the cycling result was annoying. But the...
09 July 2024 5,123 4 View
Hi everyone hope your all doing good. I’ve been looping my scripts and am trying to take a cut line of the conduction band energy with respect to y for each loop. Basically, I want to take a...
03 July 2024 9,134 1 View
Hi all, I am working on both primary hippocampal mouse neurons and i3Neurons to isolate axonal material. I plate them in microfluidic devices in house made (like any other you can find on the...
01 July 2024 8,070 0 View
Please, can the fragmentation obtained from the mass spectrometry be predicted more easily using any free software? Kindly recommend it, thanks.
01 July 2024 5,327 6 View
Is it necessary to add stop solution in Elisa plate?
30 June 2024 8,235 2 View
Basically CMOS are used for designing the SRAM cells . How the functionality will differ if we use Multi threshold CMOS instead of CMOS generally MTcmos decrease the leakage power but it will...
28 June 2024 9,845 0 View
What can be a hypothesis related to the Stock out Cost or Shortage Cost?
29 July 2024 9,259 2 View
Hi, I need to prepare a standard curve of H2O2 to evaluate the primary products of oxidation. H2O2 I have is 35%. How do I prepare a stock of H2O2 10 microlitere. If s.o has experience, I would be...
26 July 2024 3,460 2 View
Hi ResearchGate community, how are you? I need to request your help to mitigate the generation of microbubbles detected during the slide preparation of sample metaphase chromosomal spreads. I...
01 July 2024 7,694 4 View
The emerging markets have suffered from a state of stagflation. I hope you know your expectations according to your experiences regarding the implications for the stock markets,,,, risks and...
19 June 2024 4,410 3 View
I saw the iPSC about a week after holding the stock (12 well and 3 stocks) and there were very few cells attached, and some cells were differentiated. I wonder what the problem is. The plate is...
12 June 2024 6,087 0 View
I am working on antimicrobial effect of CBD (Cannabidiol). As it is insoluble in water, I prepared a stock of 500 mg/ml (in DMSO). Now I have two problems: 1- I can not dissolve more than 500 mg...
10 June 2024 9,564 6 View
Warner used to sell 8161 glass, but Harvard Biosciences bought Warner and doesn’t appear to stock it anymore
04 June 2024 5,688 2 View
My lab doesn't use phage often and recently ran into a problem with too-low of titer for our phi 11 (to the point where I couldn't get it to lyse after 3 and 24 hours). We make a lysate prep,...
27 May 2024 7,900 1 View
How can i dilute my master stock of 1 micromolar DNA template to different concentration by serial dilution
17 May 2024 3,641 2 View
Most times after checking the cyctotoxity, my results are not consistent. But i used the same concentration of my stock solution. Can anyone help out with how to determine the concentration of my...
09 May 2024 1,534 2 View