How can I keep morphology using OCT or Crymatrix in Plant vascular cambium cells?

29 December 2015 1 5K Report

Im working with fresh apical stems and this organ is very soft. After embed in Cryomatrix or OCT, it is posible to obtain around 40% of sections with good morphology (even cambium cells). But in the other 60%, shrinkage happens specially in cambium cells. Troubles:

- Samples after dry suffer of cell shrinkage, specially the cambium cells

- Samples after ethanol wash (for laser microdissection), suffer an even more shrinkage.

How could I avoid shrinkage in my cells?

Diego Yoshikay

Thanks Mohamed

Nop, ethanol is used to dry sections and fix before laser microdissectiion

Could be changed by acetone

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