I am trying to join glucose and fucose to Thrombospondin 1 (pdb code 1LSL) in order to perform a proper folding simulation. I ran a simulation using the Bevan Lab tutorial from Virginia Tech but instead of having the sugars connected to the protein and simulating the protein folding, the simulation continued with the sugars just floating next to the protein. Does anyone know how to correctly do this?

Thanks in advance,

Jordan Wolfson