thank Q madam

Microorganisms have developed mechanisms of coping with a variety of toxic metals; however, few studies have explored microbial resistance to lead.the overall mechanisms of a lead-resistant Pseudomonas marginalis and a lead-resistant Bacillus megaterium isolated from two different metal-contaminated soils were investigated..for more information read here please:http://www.ncbi.nlm.nih.gov/pubmed/10227879

It depends on which metal you are working on, to use the optimum concentration you will add to the trace mineral medium to select the tolerance isolate . 

Thank You sir 

i am going to be isolate lead tolerance organism and after isolation further how to proceed sir 

Hi Kanderi,

The lead contaminated soil itself has the microbiota which possesses resistance to the lead. The only thing you need to do is to provide it the suitable conditions in the laboratory in order to isolate and screen it.

For isolation, you can simply make serial dilutions of the lead contaminated soil and pour it over the general purpose solid medium like Nutrient Agar (for bacteria) or Potato Dextrose Agar (for fungi).

After isolation, you can further screen lead-resistance in the isolates by adding lead to the growth medium. (You need to work out the concentration of lead to be added in the medium).

These are just the preliminary steps and you need to confirm it with various biomonitoring assays.

thank you madam 

i need various biomonitoring assays for matals 

Please see the attached files/ link.

All the Best,

Karamjeet

http://link.springer.com/article/10.1007%2FBF01569930

thank Q madam