I really got confused in different protocols
how can I use: Guinea Pig Spinal Cord (GPSC) and
Complete Freund’s Adjuvant (CFA) and
Mycobacterium tuberculosis
what is the real material?
how can I prepare them?
can i use Mycobacterium Tuberculosis H37 Ra, Complete Adjuvant in Oil ( DIFCO 231131)?
Dear Dr Alavi,
In our lab, we used L-Gluthamic acid for both mouse and rats to induce EAE model of MS. Concentration was 0.5 gr/2 ml and we injected intraperitoneally 2ml/kg bw.
This model produce both behavioral and histological model for MS simialr to human.
Best regards, Good luck.
Namavar
does anybody have experiences about following method?
EAE models were established as previously described . Firstly, guinea pigs (350–450 g) were anesthetized (under 1% sodium pentobarbital, 40 mg/kg intraperitoneal injection) to obtain guinea pig spinal cord (on the ice plate) which was the main antigenic component of molding agents. Then the female rats (200–220 g) were anesthetized with 1% pentobarbital, and each rat was immunized subcutaneously with a 400 μL emulsion mixture composed of guinea pig spinal cord homogenate (1 g guinea pig spinal cord in 1 mL 0.9% saline) and an equal volume of incomplete Freund's adjuvant (Sigma, St. Louis, MO, USA) containing 10 mg/mL Mycobacterium tuberculosis H37Ra (Difco; BD Biosciences, USA) in both hind footpads and in the base of the tail. The day of immunization is designated as day 0.
Agents used for EAE induction include Pertussis Toxin (Millipore, USA),
incomplete Freund's adjuvant (Sigma, St. Louis, MO, USA), and
Mycobacterium tuberculosis H37Ra (Difco; BD Biosciences, USA)
Article Exosomes derived from mesenchymal stem cells attenuate infla...
" Li, Z., Liu, F., He, X., Yang, X., Shan, F., & Feng, J. (2019). Exosomes derived from mesenchymal stem cells attenuate inflammation and demyelination of the central nervous system in EAE rats by regulating the polarization of microglia. International immunopharmacology, 67, 268-280. "
Hello Omid,
I do not have lab experience but I did find an excellent source which describes in detail "active induction by immunization and passive induction through adoptive transfer" (Phelan, 2016). There are also visual aids and graphs as well as a list of references that might help you with your study. Since I am a medical administration student and personally have MS, I plan to take the time to read it as soon as I finish my current assignments. Thank you for alerting me to this type of study for MS and good luck!
Leese
Reference
Phelan, J. (2016, December 19). Generating EAE mouse models of multiple sclerosis. Retrieved from: https://www.taconic.com/taconic-insights/neuroscience/eae-mouse-models-of-multiple-sclerosis.html
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