We are not seeing any differential expression of EMT markers like vimentin or E/N-Cadherin in HepG2 and Hep3B cells even after 96 hours of TGFb2 (5ng/ml) and TNFa treatment (20ng/ml, in media without FBS). Prior to cytokine addition we are giving a 12 hours of serum starvation (MEM without FBS) to the cells post which we are keeping them under cytokine pressure for 96 hours in MEM without FBS. Please suggest the correct way to go.