How can I induce EMT in Hep3B and HepG2 cells by TNFa and TGFb2??

We are not seeing any differential expression of EMT markers like vimentin or E/N-Cadherin in HepG2 and Hep3B cells even after 96 hours of TGFb2 (5ng/ml) and TNFa treatment (20ng/ml, in media...

08 August 2015 2,899 3 View