Based on findings from researchers:

in mono-culture with white rot fungi, Substrate supplementation with a carbon source improves degradation of lignin. Well as, adding organic nitrogen supplement such as rice bran can increase the crude protein.

Dear Atief,

You are right, it is a good approach although it works well with only few fungi. Thank you so much for your comment.

If the objective is to produce protein then consider using the Managed Ecosystem Fermentation process. MEF specifically converts the cellulosic matter into protein biologically overnight. Attached are two papers on the subject that may be of use to you.

See the previous comment... this is the second article

Dear Edward, thank you so much for excellent reviews and fresh approaches.

With kind regards

Dear Vladimir, though you are already an expert in this area but still would like to add something from my experience.

1. if you want to increase the digestibility, optimize your procees to maximize lignin degradation and minimize cellulose and hemicellulose degaradation. It depends in which way you determine digestibility if it is through gas production test in vitro, definately the intact carbohydrate and protein will give more gas production and hence the digestibility will increase.

2. Put a experiment of SSF with fungi on water soaked and autoclaved wheat straw without any nutrient, so if fungus will not any easily accessible sugar or nutrient it will starve and switch its metabolism ti utilize lignin and a bit of cellulose and hemicellulose depending upon its nature. It could be a selective lignin degrader or simultaneous lignin degrader.

3. In y experiments Oxygen sparging helped a lot in maximizing protein and fungal biomass so try it.

Best REgards

you can use corn grit  for protein increased .

Dear researcher

you can treatment of wheat straw using tannase and white-rot fungus

You can see the link:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3974121/

Regards