I've been doing whole mount in-situ hybridization with very small embryos (~E8.5) that are fairly translucent so they're often hard to see with the naked eye. The protocol involves many washing steps so I'm constantly transferring solutions to and from the microcentrifuge tubes that we put the embryos in. Even when I'm being cautious, use a dissecting scope, and use 10µl pipette tips I still end up losing at least one embryo each time I perform the procedure.
Does anyone have methods they use for easier detection of small embryos within microcentrifuge tubes at the bench top? Laser pointers, flash lights, you name it.
Dear Davis!
Maybe You look at following article:
Article Whole-mount in Situ Hybridization of Small Invertebrate Embr...
Maybe you can find the equipment bio-laser excision ,which is autonomous （AI)
I found a protocol which uses baskets for many of the washing steps that I think I'll try! https://www.nature.com/articles/nprot.2007.514
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