I've done two different protocols with different buffers and magnetic beads but the answers were not good at all. Now I want to set up another protocol so I need more information about the pH and temperature and also the content of buffers used in every step to know what the problem is.
Another question is: Does it matter what group the magnetic surface belongs to?
Regarding the magnetic surface group, it's essential to select magnetic beads that are functionalized with ligands or molecules suitable for your application. The specific group or ligand on the magnetic surface should interact selectively with your target molecules. Common functional groups include streptavidin, protein A/G, and various antibodies. The choice of the group depends on the molecules you are trying to capture or separate.
When troubleshooting your protocol, it's a good idea to systematically examine each step, considering factors like buffer pH and composition, temperature, and the specific properties of your magnetic beads. Identifying the root cause of any issues can help you refine your protocol for better results
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