I am trying to infect E. coli O157:H7 (Sakai) using a modified M13-derived phage. The phage is able to efficiently enter phage-competent TG1 cells, but it seems to enter only 0.001% of Sakai cells (I tested a MOI of 5 and a MOI of 100 and I got similar results).

O157:H7 strains are usually able to absorb the phages, as reported in several papers.

Is there any way to improve uptake (i.e.: changing culture condition)?

Can you suggest me any reason why this phage is not entering in Sakai cells?

Similar questions and discussions