I am doing mass culture of fungus but i am getting very less amount like dry weight of fungus is 0.50-1.60 gm only because of this am getting very less amount of extract.
Hi: the biomass of the fungi depend on media type, strong medium give large amount of bio mass and weak medium give less bio mass
In the past I have cultured large amounts of biomass for secondary metabolite extraction (10litre cultures). Here is a list of things I have noticed can make a big difference to growth.
-How is the aeration of your cultures? Does your fungus have an alternate electron acceptor to oxygen?
-Is it necessary to use just PDB? It might be useful to try yeast extract, czapek dox broth or add 15% centrifuged V8 juice if this doesn't interfere with your experiment.
-Changing the trace element supplements
-Changing the inoculum load (sometime adding too many spores will cause self inhibition, but other times a strong starter culture is needed)
-Stationary vs shaking vs bubbling aeration. The secondary metabolite profile and the growth characteristic often change when the fungus is grown in a stationary liquid culture eg in a 100ml petri dish with liquid medium. But a bubbling air through a 20um filter in a flask or bioreactor often leads to a HUGE increase in biomass, but uneven growth.
-light and dark I have found will also affect some fungi and not others. but this again will affect the secondary metabolite production.
Hope this helps!
thank you oliver but i am still confuse,like weather i put my cultures on stationary or shaking for better results.
Here there are few steps; you should follow it you will get the answer of questions.
1. which fungus are you using?
2. Till date which are the available media which supports the growth of it? find it aout in literature (for e.g. PDB, Malt extract, Yeast extract and czepek dox etc...)
3. Try both static and shaking condition for all the media (mentioned in the point 2) at time and compare the growth by measuring biomass. which will give you answer of the probable growing condition for your fungal specimen.
4. what is the inoculum?
5. Maximum days of incubation. (consider the day on which you will get higher biomass production).
Best luck.
i have gone through some papers and they were also using pdb media for both condition some paper saying shaking and saying static way for 21 days.
Can you try both shaking and stationary? it varies from fungus to fungus, but also the metabolite content may change. There may be less biomass but more metabolites under a particular condition.
in shaking condition i got less biomass as well as less crude extract after evaporation.
Both PDA broth in a shaker and stationary condition for culturing various specimens of fungi vary under a particular condition. Fungal mat yield / spore load also varies under a particular condition and also depending on the fungal strain . Metabolite content varies depending on the duration of growth, media type and groth conditions etc. More extracts of metabolites up to 10-15 days duration in a liguid culture under a particular condition.
I agree with all answers mentioned, but Vipin Nagda' question was about the PDB medium, I think, it could be quit useful to try also with organic additive or adding a particular plant extract to the PDB medium, you can get profound amount of growth.
Good luck.
PDB is typically nutrient rich but you may wish to add yeast extract (1g/L) depending on the fungal strain. In addition, try slowing down the shaker speed. It may be shearing the fungal hyphae if its running too fast.
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