How long does Hela cancer cells take to reach 80% confluency after thawing from liquid nitrogen?

Three days ago, i thawed Hela cancer cells from liquid nitrogen but until now they are still suspended in the media and very very low number of cells became attached to the tissue culture...

06 January 2021 6,511 4 View

Any recommendations for a good CRISPR/Cas9 commercial system for functional genomics of fungi/yeast?

I look forward to implying a good CRISPR/Cas9 system with a selective maker for functional genomics of important genes from environmental fungi and yeast.

22 December 2020 1,432 2 View

What is the appropriate media for WI-38 cell line?

Iam going to culture WI-38 ( a normal lung cell line), it is the first time for me to deal with this cell line so i want to know the most suitable media for this cell line? In addition to, if i...

18 December 2020 6,542 3 View

Can i use an expired fetal bovine serum?

I have fetal bovine serum expired from many years ago but it kept at -200C during all this period and did not expose to cycles of freezing and thawing. So, can i use it for cell culturing? In...

18 November 2020 1,237 7 View

How can i solve the problem appeared during autogrid run using cygwin?

This is the first time for me to use the autodock software. I used autodock tools 1.5.6 I prepared pdbqt file for my ligand and my target and also file for grid parameters and docking parameters,...

02 September 2020 5,914 2 View

Which Tm should I use in my PCR ?

I have ordered primers from Thermo fisher scientific and in the analysis sheet it has been mentioned two tm The first one is tm 1M na+ 70 and the second is Tm 50mM na+ 48 and each has different...

29 August 2020 6,890 12 View

How i can Improve the accuracy of an artificial intelligence model for test data in MATLAB ?

Hello everyone, I have a question about artificial intelligence in MATLAB. Can you help me, to improve the accuracy of an artificial intelligence model for test data in MATLAB software? (while...

22 July 2020 2,692 12 View

How do you think our Architectural spaces would change after COVID 19?

How do you think our Architectural spaces would change after COVID 19?

10 May 2020 3,172 27 View

Does anyone know how can I get Mito Thermo Yellow (MTY) dye?

This is a thermosensitive dye that was recently used by chretien et al to measure changes in mitochondrial temperatures.

01 March 2020 4,863 4 View

When do you increase the number of samples?

Using the short-time Fourier transform (spectrogram) for different sample sizes

19 October 2019 3,799 2 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

Is there an open source free software for text mining and sentiment analysis?

I am looking for a free software for text mining and sentiment analysis for my research on customer review mining (it involves calculating polarity of attributes,opinion oriented information...

25 February 2021 1,920 6 View

What software programs can directly simulate Additive Manufacturing Directed Energy Deposition (DED)?

I understand there are a lot of software programs that can simulate DED by going through specific modeling, simulation, and commands. For me, that was time consuming and results were not accurate....

24 February 2021 4,428 3 View