Dear, I take a experiment about arabidopsis
And I want to get potochlorophyllide fluorescence spectrum under total dark conditions. But my plants were grown in MS plate, and when I trying to sampling that plants, light must be penetrated. So, I take fluorescence intensity, chlides that converted from pChlide intensity was measured. But many researchers detected only pchlides. But there were no specific method how they sampling the plants.
Hi,
There is not many possibilities. You must go with your plants in a dark room and prepare your samples there. To make the preparation easier, you can bring with you a weak green light. Of course, you must verify thatit does not trigger the transformation of protochlorophyllide to chlorophyllide. This is the procedure for recording 77K fluorescence spectra. For romm temperature fluorescence spectra, it is more delicate.
Of course, you cannot get a fluorescence spectrum under total dark conditions! I you are interested, I can give you access to a dropbox where are stored many papers about fluorescence measurements of protochlorophyllide. I just need your e-mail address.
Sincerely yours,
Benoît Schoefs
Dear Benoît Schoefs,
first, thank you for your kind answer. I will try to do under weak green light.
And my e-mail address is [email protected]
again, I really appreciate your attention.
Yours faithfully,
Junho Ha
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