How can I get a high yield of the bacteria?

More Nermeen Y. Abass's questions See All

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John Schloendorn

You could try to recover the plasmid from the dead bacteria by putting the glycerol stock into a mini-prep kit directly. Maybe you'll be lucky. But also maybe not...

Tom Masi

I agree with John that his suggestion may or may not work. If his suggestion does not work, you should try streaking the bacteria out on plates and then innoculate a colony that grows on the plate into liquid culture and proceed from there.

Good Luck!

Nermeen Y. Abass

Thanks all

Pablo Goldschmidt

If you wish to recover viable bacteria with high yield you should rapidly thaw the frozen aliquots at 37C adding 1 or 2 ml of broth (Schaedler or heart brain, etc) that contains 5 percent of decomplemented fetal calf sera. Preheat chocolate agar and Columbia ag ar. Plate de thawed suspension under a laminar flow to avoid cross contamination. Keep chocolate plate at 37C /5 per cent CO2 and the Columbia under anaerobic conditions. Follow growth every 24 h to assess the best growing conditions.