Nermeen Y. Abass

4 Questions 16 Answers 0 Followers

Questions related from Nermeen Y. Abass

How can I get a high yield of the bacteria?

Bacterial Glycerol Stocks for Long-term Storage of Plasmids I want to recover bacteria from glycerol stock 50% that I kept it at -80 C last 2 years ago so I tried to culture it in LB Media (pH=...

06 June 2015 7,030 4 View

Why I have some tissue give me 200 bp only and others 200 and 500 bp in RT-PCR?

I transferred cDNA to the organism that should be have this gene in their genome. Just did transfer cDNA to increase the expression of this gene. I did RT-PCR by specific primer for cDNA....

02 February 2015 6,359 10 View

The PCR product size?

I would like to be sure about this. size of the PCR product should be" 347-77= 270 or 366-77=289. I think will be 270 but need to be sure. Thanks

01 January 2015 1,352 4 View

How can I know that I have a good sequencing?

If I have about 90% homology with the gene that I did for it DNA sequencing and about 66% homology with protein. It is mean a good results? Thanks

01 January 2015 3,935 6 View