30 June 2019 1 6K Report

The slope in the equation is k/D, but it doesnt give me any reasonable value when I equate them. How can I convert the slope into size ?

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A variable moderating multiple relation - how can I test hypotheses using amos?

How can I analyse the relationship between five independent variables and one dependent variable, with a moderating variable influencing each of these five relationships? How can I test hypotheses...

03 June 2024 6,221 1 View

Any experience in fibroblast centrifugation?

We are experiencing different problems pelleting fibroblast: a) From the resuspension of the comercial vials (after defrosting, we resuspended in 5ml complete DMEM + L-Glutamin + FBS +...

28 May 2024 3,529 1 View

Fibroblast trypsinization in Hanks medium with Ca and Mg?

We are setting up fibroblast cultures in a new location and we had to change the mediums suppliers. At the trypsinization step trypsin did not work and we realized our hanks medium has Ca and Mg....

28 May 2024 6,052 2 View

Are the calibration curves of NO radical scavenging assay asymtotes?

Hello. I am here looking for help with the NO radical scavenging assay. I was working on setting up the assay with sodium nitroprusside and the Griess reagent as they do in most works. However, I...

27 May 2024 5,013 1 View

Inconsistente results with cell line LX-2, any advice?

Hi there! I'm working on my master's thesis and for my project I'm testing a new curcumin derivative in liver fibrosis. I'm working with the cell line LX-2 and have been having problems since the...

21 May 2024 2,171 0 View

Will ultracentrifugation be replaced as the most widely used method for isolating extracellular vesicles?

Ultracentrifugation is the most widely used method for isolating extracellular vesicles (EVs), despite the significant structural damage that EVs suffer during the process.

16 May 2024 9,432 1 View

¿What control can I use to inhibit the proinflammatory effect of LPS in macrophagues?

I want to evaluate the polarization to M2 phenotype in Raw Cells, I co-cultured the cell with LPS and my drug to see the effect of the drug to inhibit the proinflammatory effect of LPS, but I have...

05 May 2024 8,525 0 View

Scientific support for research in Dentistry University of Los Andes Venezuela?

Sirs. ResearchGate Receive a cordial and respectful greeting. For academic purposes only, we request collaboratively the following articles for one of our research professors, who is preparing...

05 May 2024 9,459 9 View

How can we prevent small peptides (1000 Da) from eluting out of the gel during staining in a peptide gel (tricine-SDS-PAGE, Shagger)?

How can we prevent small peptides (1000 Da) from eluting out of the gel during staining in a peptide gel (tricine-SDS-PAGE, Shagger)? There are different types of fixing solutions, but I'm not...

28 April 2024 1,522 0 View

Which are Australia regulation for disposal of produced water from oil and gas activities?

I am currently conducting research for my PhD on environmental regulations, specifically focusing on the disposal of produced water from oil and gas activities in Australia. I would appreciate any...

18 April 2024 1,544 1 View

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