Its very simple. Add water in mixture, (DMSO is highly water soluble) extract it with carbon tetrachloride or n-hexane. Your product being nonpolar will prefer to go to the nonpolar solvents. evaporate to get your product.
Its very simple. Add water in mixture, (DMSO is highly water soluble) extract it with carbon tetrachloride or n-hexane. Your product being nonpolar will prefer to go to the nonpolar solvents. evaporate to get your product.
Both the given answers are exactly correct. You follow them. One more thing as a option I want to add, which I used to do in my lab. You take small column and pack with silica gel, load the compound which is in DMSO and run the column in appropriate solvent system. The solvent system will be chosen according to the TLC.
If your product is soluble in nonpolar solvents, you can try to do exctractions whit water/hexane. Because DMSO is miscible with water.
I think Extraction will better............ u'r Pdt will be in nonpolar solvent ......use more water and take time while extracting the mixture.
Another possibility is solid phase extraction with a C18 column. Mix with water as mentioned above, pass through the column, wash with water, then wash with methanol, THF, acetonitrile, or even acetone.
It can be done if you have access to a flash chromatography system too (attached PDF).
Fist check the solubility of your product if it is soluble in ethyl acetate(ET), Then quench the DMSO containing mass in DM.water (5.0 vol) and extract it with ET . ET is better than MDC, MDC will take more impurities than ET, It may need not column, If you are getting emultion during extraction then give slow stirring and settle more time.
Try liquid-liquid extraction several times with water and a convenient solvent for your compounds, and then identify if DMSO has been completely removed from your sample.
I routinely use DMSO to extract polycyclic aromatic compounds from mineral oils. The standard procedure is to dilute the DMSO extract (200 ml) with double the volume of brine (400 ml, 4% w/v sodium chloride in deionised water) and extract the DMSO/brine solution twice with 40 ml cyclohexane (i.e. 80 ml in total). Wash the combined cyclohexane solutions twice with 25 ml portions of the 4% brine and then pass the cyclohexane solution down a short column containing 10g anhydrous sodium sulfate to remove residual water before evaporating to dryness on a rotavapor. Volumes should be adjusted pro rata. Other solvents such as pentane, heptane, dichloromethane, toluene can be used instead of cyclohexane. Always use the purest solvents available.
More volumes of water slurry is better(if your compound is insoluble in water)
Hello,
I do not know if you have a freeze-dryer but sublimation is a very powerful choice to remove DMSO from a solid.
Best regards
I agree with Irving. It will be little harder to remove all the water after washings due to the nature of the product. Recrystallize the product then sublime under mild vacuum, you will get very dry product.
Just extract with 'tons' of water several time....this works for me all the time.
Just stir with ice water for half an hour, and then leave the mixture without stirring until crystals form, then filter and wash with cold water several times
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