How can i do Crispr calculations, Please help?

John Abdo @John_Abdo3

05 February 2021 1 5K Report

I want to prepare gRNA:

I have 1.5 nmol lyophilised gRNA,

So how to prepare 7.5pmole?

Hint:

I calculated that 0.075ul contains 7.5pmol. But, it is small amount. so how can i solve this problem???

Vigneshwaran Venkatesan

If you add 15ul to 1.5 nmol, it becomes 100pM/ul.

Dilute the 100pM further to reach your desired concentration.

To dilute that, take 1ul from the stock above and add 9ul.

After 1:10 dilution, the concentration is 10pM/ul

Take 0.75ul for your experiment which corresponds to 0.75pM

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