In my opinion if you are looking for lipid histologically, you should be using frozen sections as during the processing of tissue for PE, a fair bit of lipid is lost with the xylene used. However, this may not apply to your specific purposes. That said, Oil Red-O is a good general stain for lipid, but again more effective in frozen sections and not so much in paraffin. 

I agree with Claretta, In cryostat section you can detect various lipids from unsaturated ones to  phospholipids.

Thanks Claretta and Angella for your answers, but actually I want to destroy the total tissue lipid content in a FFPE section and want to check the treatment effects further. So any suggestions for lipid destruction and quantification techniques keeping other nucleic acid or protein content as it  is?

Dear Saratupa! You must remember that histological processing of biological specimens to obtain paraffin-tissue sections include the use of solvents (ethanol, xylene) and temperature near to 56 oC to paraffin infiltration. These procedures eliminate many lipids, so will be difficult to stain it. Once you obtain tissue sections on a tissue slide try a acetone treatment or polyethylene glycol in several concentration and several times and probe Oil Red-O, SUdan stain and Osmium tetroxide. Bests regards 

Essays Biochem. 2015 Feb 15;57:81-91. doi: 10.1042/bse0570081.

Microscopy of membrane lipids: how precisely can we define their distribution?

Takatori S, Fujimoto T.

Hi Delsy,

Can you please send me the full text of the paper?

Thank you in advance

Treatment of sections with a mixture of chloroform+ethanol (3:1) for about 5 min may rid your sample of the lipids. When lipid is extracted, we expect all membranes are destroyed which compartmentalize cellular organelles and cells. It is not clear, what is the purpose of doing this? Please check relevant publications detailed at my reseachgate page, for information.

Hello Satarupa, about which aticle is interested?