by using Photoshop program and change in saturation and hue if you need one color in the image.If you need more than one color, then make a copy of your image and color each part step by step,means first color part of image for example with a blue color in the first image then use clone stamp to color the same part in the copied image then go back to the first image and change the colour to color other parts with different colours for example with green color then go back again to the copied image and use clone stamp to colour the other part with green colour.
Hello, Can't help wondering about this technique. But I love colors instead of black, white and grey. May I know which journal accept this digital colors of TEM image for publication? Thank you. All the best in your undertakings, Dr Dalia . Thank you for the tips, Dr Hanan.
BTW,
http://www.sciencemag.org/news/2016/11/see-first-color-images-produced-electron-microscope
http://www.cell.com/action/showImagesData?pii=S2451-9456(16)30357-9
If automatic segmentation worked, one could do that
and then map each area to a color palette, dependent
on the (statistical) characteristics of the
particular area.
A simple method: in Paint.net you can adjust
luminosity for each of RGB separately.
http://www.getpaint.net/doc/latest/Curves.html
Regards,
Joachim
I would not accept colored images for publication (unacceptable level of image manipulation). But for fun - why not?
You can combine images from different detectors/ acquisition conditions for RGB channels.
Article Coloring Pictures for Electron Microscopists, or Elements of...
To Mimi Sophia-Sarbandi: The Journal of Cell Biology, Cell, Frontiers in Endocrinology are few examples of journals where EM colored pictures are accepted.
One of the articles where I am coautor, containing TEM colored pictures, was published in The Journal of Cell Biology http://jcb.rupress.org/content/198/3/457. We used Photoshop to color the images.
See also https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4264519/.
Hi Dalia, not sure what you mean by coloring. Are you talking about to separate structures from the EM image? if so, it has a term, called segmentation. And there are software to do such work, of you are looking for free software, IMOD from University of Colorado at Boulder, ImageJ also has segmentation plugin, for commerical software, i used Amira, it is great software, but costy, around 4000-5000$ for one license. Hope it helps.
Hong
Hi Dalia,
I simply followed the instructions of the following video:
https://www.youtube.com/watch?v=IsUW6d1U2yM
That procedure works not only for SEM micrographs but also TEM images. As long as the original data is published as well (e.g. as supplementary data) I find false-colouring to be a powerful way to highlight structures of interest on an otherwise b&w image. Used it for a publication myself and no referee objected to it.
Best wishes,
Christian
You can use Lucis Pro for Windows or Mac to make marvelous color SEM and TEM images very quickly. Check out www.lucispro.com.
There are also YouTube videos about using this software.
Dear,
Dalia Mohamedien
You can use Photoshop software in order to colour the SEM, TEM images