we have extracted animal genomic DNA from blood and run in gel electrophoresis showed good bands, while after digesting these genome with 4 restriction enzymes showed nothing. no bands were produced everything went away.
we used
0.5ul enzyme
0.3ul bsa solution
2ul 10x enzyme buffer
5ul genomic dna
then incubated for 2 h and then run in 2% agarose gel electrophoresis directly. but there were no bands showed
I think all those enzymes may be degrading your DNA, you may try single or couple of enzyme at a time, secondly, you may also check your enzyme activity on your given sequence by using some online software and also check your enzymes in some human and animal DNA in good conc.
Adding to above, for total genomic DNA, it is not possible to get any specific bands as RE may cuts billions of times in any where. So no specfic product.
These RE in genomic DNA are used only when we need to fragment DNA to specific size, for genotyping purpose, we need to clone or PCR targeted region and use it.
thanks @Amitabh I have used single RE at time. and I used theme to genotype animal thers no problem with the activity of the RE,
I used these RE in genomic to make a diversity between breeds of sheep based on RFLP NON PCR