How can I determine the amount of RBCs in my aliquot ?

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How to solve the fluorescent dye DiI in soybean oil in the lab?

Hello together, I`m Anna, PhD-Student. The subject of my investigation ist the hydra nerve net“. We are going to stain Hydra neurons with the fluorescent dye...

06 November 2023 1,425 0 View

Anti tdTomato antibody NOT from goat for IHC?

Hi, does anyone know a working anti tdTomato antibody which works in (brain)tissue for immunofluorescent stainings? Only requirement: host cannot be goat. I´d prefer chicken but would be glad for...

10 January 2022 3,665 0 View

Is there a uniform protocol for Acceptor Photobleaching?

Hi everyone, I am currently trying to validate two fluorescent proteins as a new FRET pair by Acceptor photobleaching. As the implemented bleaching function of my microscope software (ZEN 2009)...

04 March 2021 6,806 3 View

Which tools do you use for Systems Engineering?

Using a perfect toolchain for modeling every aspect of a system is possibly a dream. But perhaps one of you can recommend a tool, that supports most systems engineering tasks.

06 February 2020 3,134 3 View

Why am I not seeing my housekeeping genes until later cycles in qPCR?

Hi all, Here is a bit of context for the question. So I took primary thymus and sliced it into 500 uM slices. I then proceeded to culture these slices for three days. After the three days, I...

05 February 2020 6,217 6 View

Suggested packages/programs for automated detection of species-specific bird vocalizations?

Does anyone have suggestions for a user-friendly, open source, automated process for identifying species-specific bird vocalizations from audio recordings? I'm currently teaching myself to use...

04 February 2020 2,552 3 View

Is CPCe's Shannon-Weaver Index processing incorrectly?

I am using CPCe with random point counts in a 1m x 1m quadrate to determine the abundance of sponge, algae, corals, and other species on the reef. I noticed that the Shannon-Weaver index was...

05 August 2019 7,126 10 View

Mediation Effects Between Groups?

I have a mediation model and have tested the effects of the model. My mediation variable does significantly and fully mediate the IV and DV. My question is can I test for significance between the...

28 April 2019 9,816 0 View

Can anyone recommend some good resource for learning identification of North American freshwater snails?

I work primarily in Arkansas. I know there's no field guide or anything that would be the snail equivalent of Merritt and Cummins Introduction to Aquatic Insects, but some regional resources for...

26 April 2018 9,185 1 View

How can I derive ionic conductivity value from measured impedance spectra of NaCl solution?

How can I derive ionic conductivity value from measured impedance spectra? I am trying to calibrate our new EIS equipment and better understand what I will be measuring. I am taking impedance...

15 February 2018 8,792 4 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

What is the best sampling strategy?

I am conducting a qualitative study that uses interviews to investigate the perceptions of teachers about a particular leadership practice and I am focusing on 3 schools which have a total number...

01 August 2024 8,457 10 View

Where to find a gene list for CRISPRa/i library screening of regulatory factors that affect pathogenic Th17 differentiation in PBMC?

I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...

31 July 2024 2,150 0 View

Can you visualize platelets using EVOS ?

Hi all, My lab has Thermo Scientific™ Invitrogen™ EVOS™ FL Auto 2 Imaging System, and I was wondering if I will be able to use it with whole blood, whilst focusing on platelets? The idea would...

24 July 2024 9,337 3 View

Culture plates for PBMC (non-adherent). For infection?

Hello, good morning. I would like to know which plate I can use to infect fresh PBMC with virus and culture for 24 hours. I have tried using 12-well and 24-well plates, but many cells adhere and...

24 July 2024 2,158 2 View

I encountered a situation where the 16s PCR amplification worked perfectly with the DNA from the same sample, but using the same sample Don't working?

I isolated microbial DNA from marine water samples taken at various sites using the Qiagen Power Water DNA Isolation Kit, including a modified enzymatic lysis step. I encountered a situation where...

24 July 2024 9,688 0 View

The best source for amplification of ADAM17 prodomain?

hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...

21 July 2024 8,660 1 View