I did DAB and NBT staining in N.benthamiana for the first time and I want to know if anyone have a protocol to quantify superoxide radical (O2 -) and hydrogen peroxide (H2O2).
An evaluated peroxide method is the TOC (total oxidant capacity) from LDN (Labor Diagnostic Nord) - Nordhorn, Germany. This method was published 2003 in Analytical Biochemistry 316:147-153 by Tatzber et al.
This may be of use: Mild episodes of tourniquet-induced forearm ischaemia-reperfusion injury results in leukocyte activation and changes in inflammatory and coagulation markers. J Inflamm 4, 12 (2007). https://doi.org/10.1186/1476-9255-4-12