What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

How can I measure a dielectric constant of ITO thin film?

Hi please guide me! I made the thin layer of ITO by using Pyrolysis spray method. but I can't find a way to measure its dielectric coefficient.(i need imaginary and real part of it) I have tried...

13 November 2020 1,465 7 View

I want to classification haplotype data by CNN. Can you introduce a suitable network?

hi everybody I converted each word into a vector with one-hot encoding. So that each haplotype has become a two-dimensional matrix. I need CNN to classify them. I appreciate your help. Best regards

25 July 2020 322 2 View

Histological scoring of acute inflammation in Trachea?

Hello, Does anyone have a detailed protocol for H&E histological scoring of acute inflammation in particularly "trachea"? Thank you. Nazli

05 July 2020 7,932 3 View

How can admixed individuals be created from different populations?

Hi everybody I want to create an admixed individuals with methods such as HI, GA or CGF. Is there a package or module in R or Python software to do this? Or software running in Windows? I...

08 April 2020 3,607 3 View

The best method to separate ethanol - water mixture?

03 April 2020 4,170 6 View

Which method most suitable for calculating similarity and genetic distance?

Hi everybody I want to calculate the genetic distance and similarity for ancestral populations. My data is haplotypes. I appreciate your help. Best regards

03 March 2020 8,142 10 View

How To Convert Snp haplotype Data Into 0,1 Matrix? Which software or package is better?

Hi everybody I want to convert HapMap Snp haplotype Data Into 0,1 Matrix so that I can use it in other works. I appreciate your help. Best regards

02 December 2019 3,312 4 View

I need a trick to remove DNA contamination from extracted RNA samples?

Hi, everyone I am extracting a total RNA from rat brain samples for real-time PCR studies, but unfortunately, there is DNA contamination in gel electrophoresis. I tried to remove it and I failed....

05 October 2019 897 6 View

The reason why rats die in targeting both substantia nigra in LPS model of parkinson's disease?

24 July 2018 7,976 3 View

How can you attach polyurethane devices on glass slides?

I am making microfluidic devices made of polyurethane. The problem I am facing is that I am unable to attach these devices to glass slide even after plasma treatment (air plasma). Some of the...

28 January 2021 5,194 2 View

How do I dissolve a TPU (Texin RxT85A) in THF?

I want to make a 6% solution of Texin RxT85A in THF. I weighed the necessary amount of Texin resin and mixed at room temperature with THF with the help of a wooden stick, this did not work at all....

18 January 2021 9,080 3 View

Is there an alternative, more ecological solvent for shape memory polyurethane other than N, N-dimethylformamide (DMF) ?

As DMF creates problems for environmental aspects, we are looking for an alternative solvent for potential commercial end use applications of SMPU.

25 November 2020 8,728 3 View

Why can`t I isolate RNA from Extracellular vesicles?

Hello, I have been trying to isolate RNA from EVs (from MSCs) for a while. I isolate the EVs using SEC. Then I use the "Total Exosome RNA and Protein Isolation Kit" from Invitrogen. When I...

21 October 2020 8,559 4 View

How can I get rid of much amount of solvent which is needed for the reaction to make film by film applicator?

Indeed, I want to make a very thin film (50 microns) of PU on the steel surface as a coat. for the reaction, much amount of CHCl3 is needed to dissolve one of the reagents to participate in the...

12 October 2020 748 3 View

What is the influence of the mold release agent on sound absorption properties of polyurethane foam ?

I'm an intern in a big company which makes flexible polyurehane foam for the automotive sector and I'm working on sound absorption properties of PU foam. I'm not chemist so I'm trying to improve...

23 September 2020 3,765 4 View

Which damage criteria should be used for nonlinear viscoelastic behavior?

Hello, I am building a material model for a nonlinear viscoelastic polymer in ABAQUS. For the beginning I just want to simulate an uniaxial tensile test. But now I want to know which damage...

13 September 2020 6,606 4 View

How can we characterize a polymer-textile substrate interface interaction?

When a textile surface such as cotton fabric is coated with a polymer with thickness approx. 20 micrometers, what will be the sample preparation, parameters, and technique to determine the...

26 July 2020 7,343 8 View

How can I extend pot life of PUR with additive?

I try to find the way to get a extend of pot life of PUR (polyurethane rigid). I use Polyol + Isocyanate (MDI) and my objetive is increase te time to work the the mixing. I use oil castor but I...

22 June 2020 9,202 11 View

How to get cel opening in a frothed PU foam?

I am making frothed PU foams (compressed air is mixed in). The foam only rises 1 to 3 mm during heating and the bubble struts of the foam tend to be strong, so they are difficult to open up. Does...

13 March 2020 2,212 4 View