I prepared co-polymer contain starch and beta-CD and I want to figure out if the co- polymer contain beta-CD or not. all the used method for beta-CD detection include interference of starch so, I want a method that is high selective for beta-CD
NMR and FTIR might not be useful as the peaks will be overlapping. I suggest DSC, as it might provide some evidence. have a look at this paper it might give away some info: Article Schiff-base based hydrogels as degradable platforms for hydr...
you might try inclusion complex formation with phenolphthalein. When this is complexed with beta-cyclodextrin the colour is lost. The inclusion is not formed with starch.
If you want to detect the existence of CLD, I suggest H NMR, which you will see the influence of CLD on starch, or starch on CLD
As alternative you may hydrolyze the starch enzymatically using amyloglucosidase and isoamylase (both from Megazyme) which convert starch into glucose, while they cannot degrade cyclodextrins.
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