I'll conduct a study among university students . First I'll stratified them according to knowledge  are "theoretical, practical and medical" then randomly chose one or two college from each area. The point is that i need to know the difference between  first and fourth year students. So after stratification can i further stratify each group to 1st and 4th year student and to take a group"cluster" from each year???

And in this stuation can i generalize the result among all university  students or should i mention that for 1st and 4th year only as they my target group??

Finally i need the help in calculation of such sample , how to calculate design effect and how to determine the size of each cluster??

Thanks in advance, 

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