I would like to perform IHC and H&E stainings on spurr (resin) embedded slides, but don't know how to remove the plastic to fully expose the tissue for optimal staining.
Try sodium-ethoxide. Prior to IHC, you must retrieve epitopes. I suggest heat-induced retrieval or citrate buffer method (the question is whether you used formaldehyde for fixation). And sometimes, depending on antigens wanted to be disclosed, there is no need to deplasticize at all (as sodium-ethoxide can destroy some of the epitopes).
Dear Michal, thanks for your helpful recommendation! I've just purchased sodium-ethoxide, which I will try it on. Can you give me more details about it? Do I have to dilute it (with what)? How long do I have to submerge the spurr embedded slide into the sodium-ethoxide solution?..., etc. I've performed MMA embedded slide successfully, which fixed with NBF. I deplasticize them with Xylene, performed heat-induced antigen retrieval with citrate buffer, then applied with antibody... Again, thanks for the help and thanks again in advance for additional details.
PS: Edited 2017-04-06,19:12 MEST for the following supplementary information on jpg. Fig. /Table 5-5-:
a) if you save the jpg you will find some hint on the original source of the table. I once in my "former, professional" life copied the table and added it to my works collection of important working SOP'S and protocols.
As of today I found a reference which might contain the table 5-5 on page 100: cf: http://onlinelibrary.wiley.com/doi/10.1002/pi.4980190342/full
BOOK review (1987) by C.A. WHITE and J.F. KENNEDY, for:
Immuno enzyme techniques in cytochemistry: W. D. Kuhlmann, Verlag Chemie, Weinheim, 1984. pp. xiii + 162
b) since I am not quite sure about the / that original source, I would like to point also to a web-file (by the same author) I found most recently:
Dear Dr. Xiaojian Li, you're welcome ! Best wishes, & good luck, WM
PS: it might also be interesting at this point (for convenience of other readers too) to mention the following "old" thread (which I was able to find in my e-data files on the matter right now): https://www.researchgate.net/post/Does_anyone_have_experience_in_extracting_DNA_from_plastic-embedded_tissue?
EDIT:
Due to the fact that the former LINK to https://www.researchgate.net .... does (DIDN'T) not work this time [2019-11-18, 14:10 UTC(+1)] I try to put another URL here (copy and paste from the original thread):