i have to set a group of positive control with DP vaccine, i find it but now i face with a big problem!! the unit of this vaccine set on LF (flocculation) , and I don't know how can I change it to mg/ml and actually this control group is too important for me to show my result and compare with it.
i read papers and protocols a lot but i found that there is no coefficient to convert it, because its not constant.
I thank you to show me solution. its really necessary. my mice is going to be older and older.
Regard.
4. CONTENTS
Country of origin of biological material: Denmark.
Liquid tetanus toxoid, non-adsorbed, was donated to NIBSC in June 2004
by Statens Serum Institute (SSI), Copenhagen, Denmark. 1 ml of toxoid
per ampoule was freeze-dried at NIBSC in November 2004, with a total of
5,900 ampoules prepared and 5,856 available for use. The material is a
purified tetanus toxoid (of purity > 1000 Lf/mg pN) stabilised with
trehalose. The average weight of the ampoule content was determined as
0.026 g of dry weight +/- 1.0%. Mean residual moisture content was
determined as 0.92%. Each ampoule contains 0.1M NaCl and 1%
trehalose.
so 1 LF = 26 mg
Hi Vasegh,
One Lf is usually equal to 2 µg of Protein as estimated by Kjeldahl Protein nitrogen determination. If you want you can estimate the Protein content of your antigen by suitable methods such as BCA or Lowry's technique.
Bye
Shiva
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