Are negative valued indicators allowed in SEM?

Hello, Using secondary and continuous data, i am trying to run a SEM with two latents. One indicator has both negative and positive values. Is it wrong? (I am gonna run the model in AMOS) Thx on...

14 February 2021 2,801 8 View

How to de-clump HEK293 suspension cells?

I have a problem with clumping of HEK293 suspension cells, and this clumping problem decreases the cell viability. I will transfect the cells if I were to reach a favorable cell count. What can I...

17 January 2021 354 4 View

How to estimate dichotomous variables in SEM?

14 October 2020 5,912 3 View

Is single indicator usage is appropriate in SEM when the data ise continous?

Hello, to use single indicator in SEM , reliability is constrained to cronbach alfa. But when we use continous data we do not calculate cronbach alfa, then can we use single indicator latents for...

02 May 2020 3,455 3 View

In structural equation, Can coefficient between covariate and indicator when controlling latent be estimated ?

Hello, In MIMIC models, can coefficient between covariate and indicator when controlling latent be estimated by direct paths from covariate to indicator? do you mind to take a look at my model...

29 February 2020 374 3 View

In SEM how we favor a model?

Hello, In structural equation modeling can we favor a model over the rival one because of better multiple correlation? I have two rival models. First is slightly have better fit values than...

16 November 2019 5,337 1 View

How many cause variables can we include in a MIMIC model?

hello, I want to run a MIMIC model in AMOS, is there a ideal number of cause indicators? Thanks on advance,

22 September 2019 4,046 9 View

Is scale mean a statistic that can be tested for significance?

Hello, My friend created a hypothesis "Is x (variable) is significant?". I found this hypothesis weird:) Anyway, he insists on testing this. I calculated the mean of the scale (x) on SPSS. Is...

31 July 2019 5,842 5 View

In SEM, can we link egzogenous observed variable to indicator of endogenous latent?

In my structural equation model, I have a an observed egzogenous variable, according to modification indices, can i link it to an indicator of endogenous latent? Thanks in advance

01 November 2018 3,191 6 View

In Amos I can not fix parameter variance to 1?

I am trying to run Second degree cfa in AMOS. As far as I know I must fix second degree factor's variance to 1. But when I right click to I can not see fixing option.I mean there is no box to fix 1.

20 July 2018 9,485 2 View

What test should i use in comparing the correlation between categorical and continuous variables?

Dear all, I have the variable (Surgery duration) represented by values in minutes and the variable (preoperative neuropraxia cases) represented as (Yes/No) values. I want to compare these two...

03 March 2021 5,427 5 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How to evaluate unsupervised clustering metrics when some elements do not have a cluster label in the result?

I run algorithms like HDBSCAN and MeanShift, and compare them with K-means and GaussianMixture. The first two algorithms do not return cluster labels for some elements in the dataset. My question...

27 February 2021 428 3 View

How can I stain the somatodendritic part of inhibitory neurons in a hippocampal culture with high specificity?

I would like to distinguish the somatodendritic part of the inhibitory neurons in a hippocampal culture (where we expect to have 5-10% of GABAergic neurons). What are the best antibodies and...

23 February 2021 6,756 1 View

Hello, does any one know a free medical text dataset?

a free multi label clinical text dataset , containing text as entry and several labels as classes. Best regards

22 February 2021 5,988 2 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

Does anyone have a working Guanidine Hydrochloride purification protocol for synthesized ssRNA?

I am trying to in vitro transcribe some fluorescein labeled ssRNA. And I want to purify the ssRNA from the free NTPs and the enzymes (RNA polymerase and DNase I). Due to the characteristic of the...

18 February 2021 4,392 4 View

How to validate image labeling quality within dataset?

Hello, it is about creating a dataset for semantic segmentation with three classes. The problem is that one class dominates with >90% and one class is

18 February 2021 4,254 5 View