Several researchers used molecular sieving through membranes. Is there any suitable method for controlling the size?
The best method is the one you described, technically it is called extrusion. It gives the perfect control over size and lamellarity, since you squeeze the vesicles through a filter with pores of a determined size.
Other methods don't give you the same degree of control, but it is nevertheless possible to achieve decent size distribution... but not a definite size. I am a fan of sonication, in case I don't have access to extrusion, which works particularly well for slightly "harder" lipids. Many colleagues of mine are happy with ether/ethanol injection. But neither can replace, IMHO, extrusion.
Proliposomes are newer technique which can be tried as an alternate if it suits for your drug.
Hi . in evaporation thin film method by increasing hydratation time, liposome get smaller in size.
good lukc...
- Badges
- Science topic
- Similar topics
- Cell Biology
- Methods