How can I construct dendogram based on Genetic distance matrix using GenAlEx?

More Maria da Glória Trindade's questions See All

FACS markers to characterize rat bone marrow-derived macrophages?

Dear all, I am looking for FACS markers to characterize my rat bone marrow-derived macrophages (M0). They were differentiated from monocytes with M-CSF. I am considering to use CD11b/c and...

15 July 2024 4,615 1 View

Do anyone experiment occasional staining problems with cryoprotectant containing Polyvinyl-pyrrolidone and Ethylene glycole?

Occasionally we observe lack of staining in the inner part of a skin section processed with indirect immunofluorescence. Can be due to incomplete removal of the cryoprotectan during the wash? Any...

07 July 2024 8,200 2 View

Dyslexia and Virtual Reality yes or no?

Do you think the use of virtual reality with dyslexia students is good?

23 June 2024 8,437 2 View

Can anyone recommend bibliography on the analysis of rage from an affect studies' perspective?

I'm analysing a novel in which the said emotion is fundamental to understand the development of characters. Thank you in advance.

22 June 2024 4,859 8 View

If I am doing a thematic analysis based on one group, is it okay to add insights from another group of interviewees?

I am doing a thematic analysis from interviewees done with my main group of interviewees. I also have done two interviews with another group of interviewees which provided interesting insights...

21 June 2024 3,305 6 View

¿En qué medida las tecnologías educativas están siendo efectivas para promover el desarrollo de habilidades para la vida?

las

19 June 2024 2,125 3 View

Is there any way to avoid albumin contamination in isolated extracellular vesicles?

Hi, I am doing western blotting of isolated extracellular vesicles, and I have albumin and calnexin contamination that I don't know why. Is there any way to avoid it?

16 June 2024 9,907 4 View

Is it okay to structure the results section of a thematic analysis based on your research questions?

I am going into my inductive thematic analysis with three research questions. In the results section, is it okay to structure it according to these three research questions, presenting the themes...

16 June 2024 1,116 3 View

How can I add my old article to my profile, if I am the first author, but I have different name before marriage?

Please note, that this is my first manuscript under the name Mária Čápová. Then I have been married, and my name change to Plachá, but I would like to have this article in my profile. Ontogenetic...

16 June 2024 7,178 0 View

Can I design a probe for taqman qpcr in antisense?

I have a target of sequences for primer design for qPCR with taqman. The issue is that when I design a sense probe the probe makes dimers with forward and reverse with a very high probability at...

16 June 2024 1,506 1 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Does soybean seed coat or cotyledon contain chlorophyl or flavonoids? What types? determined by paper chromatography? other methods (high school lab)?

Am trying to develop a lesson/lab to determine and compare antioxidant properties of soybeans of various colors. Preference would be low tech and low cost. Any assistance is greatly appreciated.

25 July 2024 7,498 2 View

How do you assign allele numbers and sequence type after amplifying housekeeping genes by PCR for MLST?

How to assign allele numbers and sequence type after amplifying housekeeping genes by PCR for genotyping of S. pneumoniae strains by MLST using bioedit ?

19 July 2024 5,239 2 View

Promotor observation in region annotation of RNA RIP-seq?

Hello all, I extracted RNA from my samples and performed RIP-seq. After annotating the genomic regions using R, I obtained promoters, exons, introns, and UTRs. Given that my samples consist of...

18 July 2024 1,579 2 View

Why does it show two bands when I add single hairpin structures to a gel?

Hi everyone, I am working on a hybridization chain reaction (HCR) and trying to visualize it with gel electrophoresis. The visualization of the HCR works but for the single components of this...

07 July 2024 9,413 4 View

Can I use soybean, sunflower, or olive oil to prepare a standard curve for lipid estimation in plants or pollen?

I am trying to analyze lipids in plants; specifically pollen by using Sulfo-Phospho-Vanillin (SPV) Method! Can I prepare a standard curve for this estimation using soybean, sunflower, or olive...

01 July 2024 3,267 1 View

Why and how to solve unmatched forward and reverse sequencing result by Sanger sequencing?

I’m currently working on polyglutamine disease by Sanger sequencing to count the CAG repeats. However, since my targeted genes involve CAT or CAA interruption in the CAG repeats, their...

01 July 2024 8,328 5 View

Is defatted soybean considered a pulse?

According to various research, soybean is not considered a pulse because of its high fat content.

30 June 2024 6,311 0 View

Abhijeet Singh

https://www.researchgate.net/post/How_to_generate_the_phylogenetic_tree_if_I_have_distance_matrix_rather_than_alignment

Mostafa Haghpanah

Dear Maria da Glória Trindade

send me your raw data

Maria da Glória Trindade

Dear Dr. Haghpanah, see annex the file. Thank you very much for your attention.

dear Maria da Glória Trindade

here you are

similarity matrix = Jaccard

algorithm of dendrogram = UPGMA

Thank you very much! It is exactly what I need.

Greetings from Brazil!

Dear Ahmad Al-Saleh

I used Ntsys software (trial ver)

Mehmet KOÇ

you can use this web site. free and so easy

http://genomes.urv.cat/UPGMA/index.php?entrada=Example2

Thank you Mehmet KOÇ . I am going to try it.