How can I compare the cell yields (biomass) of aerobic and anaerobic digestion?

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What is best extraction method to extract benzene from water?

What can be the best and precise extraction method that can be used to extract benzene from water in order to measure it my GC-FID,

01 February 2018 4,174 8 View

Hi, Good evening all, can anyone suggest me some membranes which are having a porosity less than 0.22 micrometers?

here, I am looking for membrane that has a porosity less than 0.22 micrometer which can allow the diffusion of solvents through it with blocking the moments of microorganisms? any suggestions are...

11 December 2016 2,740 5 View

FTIR spectroscopy analysis for membrane fouling?

When a fouled membrane like Nafion was analyzed for FTIR analysis, before mesurment should we remove the flock (layer of bacterial biofilm) on the membrane or not? For the assessment of membrane...

06 July 2014 6,182 2 View

What is meant by 20% NaOH solution, how can I prepare it?

How can I prepare 20% NaOH solution from powdered NaOH? 20 g+80 ml water or 20 g in 100 ml? Or any other suggestions?

31 December 2013 12,397 18 View

Can anyone please tell me, how to detect or find any unknown compounds (like organics, carbohydrates or some enzymes secreted by algae.) present in water samples?

09 October 2012 1,500 6 View

Is there any cases algae not using the nutrient from the wastewater and grow normally?

I am working on microalgae cultivation using waste water. The initial concentration of nutrients were less but the microalgae has achieved biomass growth of 2 g/L. The final concentration of...

08 August 2024 4,812 2 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...

05 August 2024 4,945 3 View

Recovery rate after peptide desalting using Sep-pack C18 1 cc Vac Cartridge?

Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?

31 July 2024 6,544 11 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

Question about MHB (Mycorrhiza Helper Bacteria) isolation technique??

What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.

26 July 2024 7,387 0 View

How to remove cell debris after tissue dissociation?

I'm facing issues with the tumor dissociation protocol for GBM. I use collagenase/dispase with DNase to dissociate my tumor sample. However, even after incubating it for 60mins, I do not get a...

22 July 2024 412 3 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View