I am planning the sorting of cancer stem cells from blood. There are more than 10 cell surface antigens. How can I choose the best ones to get the highest sensitivity and specificity?
Hello, I will isolate circulating ovarian cancer stem cells from human blood. I considered several methos which are magnet based stem cell isolation kits, flow cytometry, immunohistochemistry....
02 March 2018 3,040 4 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
I went for Plackett Burman Design for selecting the optimization factors of growth conditions for pesticide degradation. I started with 7 parameters. But, after the experiments and analysis, only...
29 July 2024 5,015 1 View
In hirshfeld surface calculation i did not find any red spot in hirshfeld surface, is it ok for research article to write?
28 July 2024 2,988 0 View
Dear researchers, Why specific capacitance is not proportional to the specific surface area? I have material A with a surface area higher than material B, but material B is showing little bit...
26 July 2024 9,156 2 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View
Hi everyone, I have a question regarding surface and interfacial tension. Although I understand that these tensions are studied for different purposes, can they be considered equivalent in the...
18 July 2024 382 5 View