Sorry this is not an answer but two questions. What is "NCS"?? What NCS are you preparing from which amine?? You have to be more specific.

It is amazing how many people use TLA's. Do you know what a TLA is??

TLA = Tthree Letter Abbreviation!!

hey Hans,

NCS meaning group isothiocyanates.. N=C=S group

I think, NCS is very general term used for isothioyanates

TLC means thin layer chromatography. It is also very common abbreviation used in organic chemistry.

I want to know stain used to detect isothiocyanate group.

Thanks for your interest.

Have a nice time.

ITC isolate (dichloromethane (DCM) extracts) detection on TLC, silica gel 60 F254 plates (Merck, Germany): can be done after thiourea derivatization. In order to prepare thiourea, 0.5 ml of ethanol and 0.5 ml of amonia (25%) add to the concentrated DCM extracts. Keep the solutions kept in refrigerator for 12 h. Apply the thiourea derivatives on TLC (silica gel G60 F254) with the lower phase of solvent system of ethyl acetate:chloroform:H2O (40:30:30). After developing and drying, the plates spray with Grote's reagent which is adequate for identification of thiourea derivatives by blue color spots.

GROTE'S REAGENT

Use: Reagent for sulfur in organic compounds.

Preparation: Dissolve 0.5 g. of sodium nitroferricyanicle in 10 ml. of water, and add 0.5 g. of hydroxylamine hydrochloride and 1 g. of sodium bicarbonate. Wait until a gas is no longer evolved and then add 2 drops of bromine. Filter the dark solution and make up to 25 ml.

Procedure for Test: Dissolve a few mg. of the substance under investi-gation in a little water, and add an excess of sodium bicarbonate and 0.5 ml. of the reagent. If a sulfur compound is present, a purple-red color forms within 10 minutes.