If i want to do my research data analysis what i should start with?

like a guide especially with putting the score for my survey. *my survey will measure knowledge level for the disease population Moreover, then which tool should I use? My study is a...

18 January 2021 3,819 10 View

Can I use 24 wells black tissue culture uncoated plate for cultuing macrophage (THP-1 treated with PMA) ?or I have to coat them?

Hello, I will start an experiment using macrophage (THP-1 treated with PMA). I have ordered the plate that suitable for fluorescence plate reader which is glass 24 wells black uncoated tissue...

05 January 2021 4,214 3 View

What is the ideal cell number for detection of HIF using ELISA ?

Hi All, I am planning to measure HIF expression with different cellular conditions using ELISA kit, from your experience what is the ideal number of cells used for this type of experiment?

07 December 2020 3,066 3 View

Why I got different sizes with polyacrylamide nanoparticles when I measure the size by DLS? I've prepared them with same procedure?

I've prepared nanoparticles by inverse microemulsion technique and I've functionalised the nanoparticles with N-(3-aminopropyl)-methacrylamide (APMA) (+ve charge) to facilitate cellular delivery,...

02 December 2020 8,329 3 View

My protein ladder is diffused and take a lot of time for running electrophrosis?

hello, I am doing western blot for cancer cell and my protein ladder diffused during running electrophoresis and the sample is disappear. also it takes too much time more than 3 hr for migration....

14 September 2020 5,113 4 View

How can I quantitatively analyze a sample with carbohydrate content?

Can anyone -who may have hands-on experience in analytical chemistry or photochemistry- help me how can I quantitatively analyze a sample with carbohydrate content?

09 July 2020 3,663 2 View

How to model VLC V2V communication system in matlab?

I want to simulate what will be BER vs SNR if i sent a stream of bits through VLC in outdoor system; V2X or V2V. any help??

09 February 2020 6,478 4 View

How to remoe imaginary part of Zin in hfss?

i am working on 6 MHz antenna and i want to remove the imaginary part in the input impedance. i used Lumped RLC bounday condition but no sol. any help?

11 September 2018 2,320 3 View

How to import mimics file to ANSYS workbench?

17 August 2016 4,200 4 View

Is it feasible to do cell viability test by counting cells using trypan blue in a 96-well plate? How can I detach cells from wells exactly?

02 January 2016 5,165 6 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

What is the density for pva electrospining?

I'm Juan Carlos, a high school student who is working with electropinning, I made an electropinning machine with a 21g needle and a 30kv high voltage source and the distance between my needle is...

28 February 2021 298 3 View

Is that possible to do an impedance measurement without FRA module?

One of my colleagues said, "the impedance you can measure with the three electrodes and with the specific sequence varying the frequency" Is it possible to configure the potenciostat without the...

21 February 2021 418 2 View

Choice of Ab for Co-IP: Does an anti-(tag) antibody (such as anti-flag/ anti-myc) a must if I am using co-transfected cells for Co-IP?

Hi guys, I am trying to detect the binding of 2 proteins by Co-immunoprecipitation. However, the choice of antibody is confusing me. I tried probing the endogenous protein right away but the...

16 February 2021 9,050 5 View

How to show in a scientific paper format the post-hoc test of 4 variables involved, when 3 by 3 variables are significant?

In a study involving 4 variables (species(2x), temperature(2x), nutrients(2x) and light(3x)), 3 by 3 variables, for example, "species", "temperature" and "nutrients" are significant. I end up with...

15 February 2021 8,018 2 View

Why are we measuring gel content of NBR latex? Does gel content related to the amount of cross-linked in a polymer?

Hi good day to everyone! I am actually studying the gel content of synthetic latex. Refer to ASTM 3616, gel content is measure of insoluble rubbers in the chosen solvent. So, I just would like to...

07 February 2021 605 4 View

How to mesh complex geometry?

I have been trying to mesh some complex geometry and have been having issues. I have attached images of the geometry in question. This structure is very large in size but has some comparatively...

05 February 2021 6,379 5 View

Can anyone help me with the problem of hyperelastic coefficients in abaqus?

Hello Everyone, I have done a uniaxial test on rubber and data (attached with the post) has been used in Abaqus to get YEOH's coefficient. But I am getting a warning that it is unstable? Can...

04 February 2021 5,975 3 View

STS Score - Excel sheet calculator?

I'm looking for an excel sheet to calculate the STS Score. From the website http://riskcalc.sts.org/stswebriskcalc/calculate. Does anyone have a worksheet to calculate the STS Score?

25 January 2021 958 4 View

How can I do antigen retrieval for proliferative markers (MCM2, Ki67 etc) While preserving a sensitive endogenous reporter?

I am trying to do antigen retrieval on mouse brain sections to stain for proliferative markers like MCM2, Ki67, PCNA etc on tissue that has mCherry conjugated to a transcription factor of interest...

18 January 2021 1,023 3 View