I'm trying to analyze RT-PCR data using the Pfaffl method. I'm using a template which is available online and it is here attached.

When calculating the fold induction for triplicate (the last spreasheet from the excel), my control group is always 1 and thus the SD would be 0. Is there any way to calculate SD for control based on Pfaffl method and using this template?

I've also looked at other related questions here on researchgate and have also tryed other templates but the graphs and SD are very diferentt.

Thanks in advance.