3 Questions 8 Answers 0 Followers
Questions related from Luis Félix
Dear all, We are trying to quantify different proteins in 6 dpf zebrafish larvae. After exposure we collect the larvae in PBS which is then replaced by RIPA buffer containing PMSF and a cocktail...
03 February 2022 782 2 View
Hi, I'm performing western blots on different samples and I'm facing a some problems after eletrotrasnfer with PVDF membranes. In general, during the incubations with the primary and secondary...
19 November 2020 6,723 7 View
I'm trying to analyze RT-PCR data using the Pfaffl method. I'm using a template which is available online and it is here attached. When calculating the fold induction for triplicate (the last...
19 May 2015 7,497 2 View